RESUMO
In the present work the effects of the hypophysis hormones on oviduct mucoprotein components distribution patterns were studied. Remarkable changes after treating the toad with hypophysis injections were apparent. The distribution pattern for hexose, sialic acid, hexosamine and phosphate from 18 hours hypophysis treated toads were found to be identical with those obtained from preovulatory period animals. On the other hand, the levels for mucoprotein components from hypophysis treated animals were found to be approximately one-half or more higher than those obtained from postovulatory period toads. Otherwise, hypophysis treatment of the toads in preovulatory period had not effect on the levels and distribution patterns of mucoprotein components. These results suggest that hypophysis hormones are involved in the increase of the oviduct secretory activity.
Assuntos
Mucoproteínas/metabolismo , Oviductos/metabolismo , Hormônios Hipofisários/farmacologia , Animais , Bufo arenarum , Feminino , Hexoses/metabolismo , Oviductos/efeitos dos fármacos , Ovulação , Fosfatos/metabolismoRESUMO
In the present work the effects of the hypophysis hormones on oviduct mucoprotein components distribution patterns were studied. Remarkable changes after treating the toad with hypophysis injections were apparent. The distribution pattern for hexose, sialic acid, hexosamine and phosphate from 18 hours hypophysis treated toads were found to be identical with those obtained from preovulatory period animals. On the other hand, the levels for mucoprotein components from hypophysis treated animals were found to be approximately one-half or more higher than those obtained from postovulatory period toads. Otherwise, hypophysis treatment of the toads in preovulatory period had not effect on the levels and distribution patterns of mucoprotein components. These results suggest that hypophysis hormones are involved in the increase of the oviduct secretory activity.
RESUMO
In the present work the carbohydrate utilization by the avian oviduct during the egg formation was studied. The pattern obtained for total hexose, fucose, hexosamines, sialic acid, fructose and sulphate demonstrated that in the domestic fowl's oviduct the isthmus and the shell gland secrete the precursors for the protein carbohydrate substance of the mammillae and shell matrix and may contribute during the shell formation with the substrates necessary for the metabolic energy of the organ. The infundibulum only participates as a donor for the metabolic energy.
Assuntos
Metabolismo dos Carboidratos , Galinhas/metabolismo , Oviductos/metabolismo , Oviposição , Animais , Feminino , Frutose/análise , Fucose/análise , Hexosaminas/análise , Hexoses/análise , Oviductos/análise , Oviductos/anatomia & histologia , Ácidos Siálicos/análiseRESUMO
In the present work the carbohydrate utilization by the avian oviduct during the egg formation was studied. The pattern obtained for total hexose, fucose, hexosamines, sialic acid, fructose and sulphate demonstrated that in the domestic fowls oviduct the isthmus and the shell gland secrete the precursors for the protein carbohydrate substance of the mammillae and shell matrix and may contribute during the shell formation with the substrates necessary for the metabolic energy of the organ. The infundibulum only participates as a donor for the metabolic energy.
RESUMO
In the present work the carbohydrate utilization by the avian oviduct during the egg formation was studied. The pattern obtained for total hexose, fucose, hexosamines, sialic acid, fructose and sulphate demonstrated that in the domestic fowls oviduct the isthmus and the shell gland secrete the precursors for the protein carbohydrate substance of the mammillae and shell matrix and may contribute during the shell formation with the substrates necessary for the metabolic energy of the organ. The infundibulum only participates as a donor for the metabolic energy.
RESUMO
A histo-biochemical correlation was carried out in oviducts of Bufo arenarum (Hensel), in trophic animals in the preovulatory period. Histochemical techniques were used to detect acidic mucoproteins, whereas complex mucopolysaccharides were biochemically assayed, thus simplifying the histologic methodology. The existence of acetylhexosamines, hexoses, phosphate and sialic acid in the oviduct was put in evidence through histochemical and biochemical methods. Results show a high content in sialic aicd, total phosphate and acetylhexosamines in the middle and terminal portions of the preconvoluted zone. Sialomucins, acetylhexosamines and hexosamines show a maximum in the last loops of the convoluted zone, coincident with clearly determined histologic areas. All components detected exhibit minimal values in the proximal and distal intermediate zones. Results found for phosphate show it is combined, and determines the reactivity to the stains used. This type of mucin has not been found in other species.
Assuntos
Bufo arenarum/metabolismo , Oviductos/citologia , Animais , Feminino , Hexosaminas/análise , Hexosaminas/metabolismo , Hexoses/análise , Histocitoquímica/métodos , Oócitos/análise , Oócitos/metabolismo , Oviductos/anatomia & histologia , Oviductos/metabolismo , Fosfatos/análise , Ácidos Siálicos/análise , Ácidos Siálicos/metabolismoRESUMO
A histo-biochemical correlation was carried out in oviducts of Bufo arenarum (Hensel), in trophic animals in the preovulatory period. Histochemical techniques were used to detect acidic mucoproteins, whereas complex mucopolysaccharides were biochemically assayed, thus simplifying the histologic methodology. The existence of acetylhexosamines, hexoses, phosphate and sialic acid in the oviduct was put in evidence through histochemical and biochemical methods. Results show a high content in sialic aicd, total phosphate and acetylhexosamines in the middle and terminal portions of the preconvoluted zone. Sialomucins, acetylhexosamines and hexosamines show a maximum in the last loops of the convoluted zone, coincident with clearly determined histologic areas. All components detected exhibit minimal values in the proximal and distal intermediate zones. Results found for phosphate show it is combined, and determines the reactivity to the stains used. This type of mucin has not been found in other species.
RESUMO
A histo-biochemical correlation was carried out in oviducts of Bufo arenarum (Hensel), in trophic animals in the preovulatory period. Histochemical techniques were used to detect acidic mucoproteins, whereas complex mucopolysaccharides were biochemically assayed, thus simplifying the histologic methodology. The existence of acetylhexosamines, hexoses, phosphate and sialic acid in the oviduct was put in evidence through histochemical and biochemical methods. Results show a high content in sialic aicd, total phosphate and acetylhexosamines in the middle and terminal portions of the preconvoluted zone. Sialomucins, acetylhexosamines and hexosamines show a maximum in the last loops of the convoluted zone, coincident with clearly determined histologic areas. All components detected exhibit minimal values in the proximal and distal intermediate zones. Results found for phosphate show it is combined, and determines the reactivity to the stains used. This type of mucin has not been found in other species.
